2024
Medical Microbiology II
Name: Medical Microbiology II
Code: MVT14029I
3 ECTS
Duration: 15 weeks/78 hours
Scientific Area:
Veterinary Medicine
Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Presentation
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Sustainable Development Goals
Learning Goals
The learning objectives are:
• To know the aspects of the biology and pathogenesis of viruses, in order to establish the most appropriate control strategies.
• To know the various types of viruses with Veterinary importance.
The skills to be developed are:
• Ability to manipulate laboratory instrumentation and the various techniques in the treatment of clinical samples containing viruses;
• Ability to plan and perform virological tests;
• Ability to produce technical reports.
• To know the aspects of the biology and pathogenesis of viruses, in order to establish the most appropriate control strategies.
• To know the various types of viruses with Veterinary importance.
The skills to be developed are:
• Ability to manipulate laboratory instrumentation and the various techniques in the treatment of clinical samples containing viruses;
• Ability to plan and perform virological tests;
• Ability to produce technical reports.
Contents
Theoretical
1. Origin of viruses. Virology as a science.
2. General properties of viruses.
3. Genome and genomic organization. Structural and non-structural proteins.
4. Taxonomy.
5. Replicative cycle. Viruses with DNA genome. Viruses with RNA genome.
6. Exit routes of host cell.
7. Synchronized replication cycle - one-step replication cycle.
8. Viral genetics: Recombination and reassortment.
9. Viral oncogenesis.
10. Viral pathogenesis: Interactions between host and virus.
11. Immune response to viral infections.
12. Virulence. Streaming. Epidemiology. Types of infection.
Practical
Molecular methods of virus detection and identification.
Inoculation of eggs.
Rapid hemagglutination.
Titration of hemagglutinating viruses by the micromethod.
Inhibition of hemagglutination (HI).
Cell culture.
Titration of viruses in cell culture.
Cytopathic effect and determination of TCID50.
Formation of lysis plates and determination of 100 PFU.
Viral neutralization and seroneutralization.
1. Origin of viruses. Virology as a science.
2. General properties of viruses.
3. Genome and genomic organization. Structural and non-structural proteins.
4. Taxonomy.
5. Replicative cycle. Viruses with DNA genome. Viruses with RNA genome.
6. Exit routes of host cell.
7. Synchronized replication cycle - one-step replication cycle.
8. Viral genetics: Recombination and reassortment.
9. Viral oncogenesis.
10. Viral pathogenesis: Interactions between host and virus.
11. Immune response to viral infections.
12. Virulence. Streaming. Epidemiology. Types of infection.
Practical
Molecular methods of virus detection and identification.
Inoculation of eggs.
Rapid hemagglutination.
Titration of hemagglutinating viruses by the micromethod.
Inhibition of hemagglutination (HI).
Cell culture.
Titration of viruses in cell culture.
Cytopathic effect and determination of TCID50.
Formation of lysis plates and determination of 100 PFU.
Viral neutralization and seroneutralization.
Teaching Methods
Per week there will be a lecture (60 min) and a practical (120 min).
The lectures will be plenary dedicated to the acquisition of concepts and basic knowledge. The practical classes will be practical work developed by students after exposure by the teacher.
Continuous assessment - 2 tests minimal score equal to or higher than 10 (9.5) in each of the tests.
Exam - written exam. minimal score equal to or higher than 10.
Whatever the chosen regime, students will have to take a practical test, in which they must obtain a minimum grade of 10.
Students who have not attended the minimum 75% of practical classes will have to take an extra exam with a minimum grade of 10. The practical grade will be the arithmetic mean of these two grades.
The final grade 50% theoretical grade and 50% practical grade.
In the Special Season and Extraordinary Season the exam is oral, including theoretical and practical subjects.
The lectures will be plenary dedicated to the acquisition of concepts and basic knowledge. The practical classes will be practical work developed by students after exposure by the teacher.
Continuous assessment - 2 tests minimal score equal to or higher than 10 (9.5) in each of the tests.
Exam - written exam. minimal score equal to or higher than 10.
Whatever the chosen regime, students will have to take a practical test, in which they must obtain a minimum grade of 10.
Students who have not attended the minimum 75% of practical classes will have to take an extra exam with a minimum grade of 10. The practical grade will be the arithmetic mean of these two grades.
The final grade 50% theoretical grade and 50% practical grade.
In the Special Season and Extraordinary Season the exam is oral, including theoretical and practical subjects.
Teaching Staff (2023/2024 )
