Biochemical Analysis I

Code: QUI00344L

6 ECTS

Duration: 15 weeks/156 hours

Scientific Area: Biochemistry

Teaching languages: Portuguese

Languages of tutoring support: Portuguese

Presentation

Experimental design, methods of qualitative/quantitative analysis of biomolecules: differential spectrometry, fluorescence, centrifugation, electrophoresis and high-performance chromatography.

Sustainable Development Goals

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Vida terrestre - Proteger, recuperar e promover o uso sustentável dos ecossistemas terrestres, gerir de forma sustentável as florestas, combater a desertificação, deter e reverter a degradação da Terra e deter a perda da biodiversidade.

Learning Goals

1.To know and understand theoretical and experimental information in domain of analytical biochemistry that include terms, concepts, factors, principles, laws, theories, methods and procedures related with experimental design, control of quality results, centrifugation, electrophoresis, HPLC and GC techniques, differential spectrometry and fluorescence in biochemistry. 2.To develop a critical aptitude to equate new problems in domain of analytical biochemistry which allow a conception of new experimental strategies. 3. Analysis and control of quality of biochemistry assays. 4.Execute, in accurate form experimental procedures to obtained results and its interpretation and discussion. 5.To develop capacity to prepare scientific reports and to organize team work laboratorial planes, which improve the knowledge in analytical biochemistry domain, useful for prepare scientific communications and laboratorial management.

1. Experimental design and control of quality.
2. Applications of differential spectrometry and fluorescence in qualitative and quantitative analysis of biological molecules.
3.Centrifugation, analytical, preparative and gradient centrifugation. Subcellular separation and enzymatic localization.
4.Electrophoresis, matrix and buffers, electric parameters, PAGE, IEF e 2D. Equipment, sample preparation, application and run. Gel staining and conservation.
5.HPLC, mobile and stationary phases, solute retention mechanisms. Experimental optimization. Chromatograph, columns, detectors, hyphenates techniques.
6. GC, mobile and stationary phases, solute retention mechanisms, columns technology. Chromatograph, temperature, injectors and detectors. Data acquisition. Column and carrier gas supply. Flux programming of carrier gas.

Teaching Methods

1.The lectures are plenary lessons structured in the scientific method which combine subject exposition and bibliographic-assisted search to generate capacities to understand strategies and to apply methodologies which allow executing experimental protocols with biochemistry application. 2. The practical classes take place in teaching laboratories where student teams execute laboratory-assisted work following protocols previously supplied by the teacher or delineated by the student resorting to bibliographic information. Also will teach instructions to write a report. 3. Tutorial classes involve instants of individual contacts or in little teams occurring search, read and assisted-interpretation of scientific papers which contribute to preparing experimental assays and the project. Assessment methods: theoretical component: written examen (40%), project (monograph/oral presentation and discussion (30%); practical component: experimental work and report (30%)