2023
Nucleic Acids Biochemistry Lab
Name: Nucleic Acids Biochemistry Lab
Code: QUI12399L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biochemistry
Teaching languages: Portuguese
Languages of tutoring support: Portuguese, English
Regime de Frequência: Presencial
Presentation
Laboratory approaches that illustrate nucleic acid manipulation for cloning applications, analysis of gene expression, detection of pathologies, gene therapy and obtaining of GMOs
Sustainable Development Goals
Learning Goals
1.To know, to understand and to valorise the concepts of structural and functional genomic, as well as, manipulation techniques of nucleic acids and its application in development studies, molecular evolution, regulation of gene expression, vegetal and animal molecular pathologies, gene therapy and construction of OGMs.
2.Give capacity to identify nucleic acids and to infer its purification state; to delineate and execute experiments that analyse DNA and/or RNA which improve the knowledge about genome and proteome of living organisms, respecting ethic and biochemical deontology. Give capacity to organize team work laboratorial planes and execute, in accurate form, experimental procedures which contributes to detect gene, microbial and forense characteristics, to delineate therapeutic assays, to obtain OGMs and to search nucleotide and protein sequences, using bioinformatics as complement of experimental results.
2.Give capacity to identify nucleic acids and to infer its purification state; to delineate and execute experiments that analyse DNA and/or RNA which improve the knowledge about genome and proteome of living organisms, respecting ethic and biochemical deontology. Give capacity to organize team work laboratorial planes and execute, in accurate form, experimental procedures which contributes to detect gene, microbial and forense characteristics, to delineate therapeutic assays, to obtain OGMs and to search nucleotide and protein sequences, using bioinformatics as complement of experimental results.
Contents
1. Extraction of nucleic acids from different biological materials, viral, bacterial and plasmid DNA, nuclear and organelles DNA; RNA and mRNA .
2. Utilization of restriction endonucleases, DNA ligases and DNA polymerases.
3. Restriction maps and DNA sequencing.
4. Polymophisms detection, RFLP, RAPD and microssatelites.
5. DNA Polymerisation chain reaction (PCR), detection and amplifying of specific sequences. Technical modifications of PCR: RT-PCR, quantitative PCR and real-time PCR.
6. DNA clonning, vectors, host cells, construct and analysis of DNA and cDNA libraries.
7. Gene expression, analysis of genetic expression by northern and western blotting and RT-PCR.
8. Clonning and heterologe expression of gene sequences.
9. OGMs and detection of OGMs in food using PCR.
10. DNA technology in industry, medicine, agriculture and research. Ethic and risks.
2. Utilization of restriction endonucleases, DNA ligases and DNA polymerases.
3. Restriction maps and DNA sequencing.
4. Polymophisms detection, RFLP, RAPD and microssatelites.
5. DNA Polymerisation chain reaction (PCR), detection and amplifying of specific sequences. Technical modifications of PCR: RT-PCR, quantitative PCR and real-time PCR.
6. DNA clonning, vectors, host cells, construct and analysis of DNA and cDNA libraries.
7. Gene expression, analysis of genetic expression by northern and western blotting and RT-PCR.
8. Clonning and heterologe expression of gene sequences.
9. OGMs and detection of OGMs in food using PCR.
10. DNA technology in industry, medicine, agriculture and research. Ethic and risks.
Teaching Methods
1. In this unity are teach a little number of lectures, plenary lessons structured in scientific method which combine subject exposition relatives to technical aspects of nucleic acids biochemistry with bibliographic-assisted search to expand complexes subjects, which are illustrated in laboratorial classes.
2.The practical classes take place in teaching laboratories where the students execute laboratory-assisted work following protocols, previously supplied by the teacher or delineated by the student resorting to bibliographic information, which involve extraction and DNA/RNA handling. Tutorial classes involve instants of individual or in little team contacts occurring search, read and assisted-interpretation of scientific papers which contribute to prepare its oral presentation and protocols creation.
Assessment methods:
Theoretical component: oral presentation of two scientific papers (40%)
Practical component: Experimental work (30%) and report (30%)
2.The practical classes take place in teaching laboratories where the students execute laboratory-assisted work following protocols, previously supplied by the teacher or delineated by the student resorting to bibliographic information, which involve extraction and DNA/RNA handling. Tutorial classes involve instants of individual or in little team contacts occurring search, read and assisted-interpretation of scientific papers which contribute to prepare its oral presentation and protocols creation.
Assessment methods:
Theoretical component: oral presentation of two scientific papers (40%)
Practical component: Experimental work (30%) and report (30%)
Teaching Staff
- Isabel Maria Simão Alves Pereira Ferreira [responsible]
