2024
Genetic Engineering and Biotechnology
Name: Genetic Engineering and Biotechnology
Code: CMS12239L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biological Sciences
Teaching languages: Portuguese
Languages of tutoring support: Portuguese, English
Regime de Frequência: Presencial
Sustainable Development Goals
Learning Goals
1.Understanding new Biotechnologies from the view point on integration of multiple scientific concepts of
Biological Sciences for the specific molecular manipulation of living organisms.
2.The teaching will be directed for the familiarization of techniques for the creation of added value from
new biotechnological processes and products.
3.Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology,
concepts for application of the new techniques will be discussed.
4.Application of Molecular Biotechnology on the Health (active substances, vaccines, diagnostic
techniques and gene therapy); on the Environment (Toxic elimination and bioremediation); and on the
Agriculture (transgenic plants and animals for the food chain).
5.Laboratory work will be focused on the most common techniques used for the most recent
biotechnological applications
Biological Sciences for the specific molecular manipulation of living organisms.
2.The teaching will be directed for the familiarization of techniques for the creation of added value from
new biotechnological processes and products.
3.Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology,
concepts for application of the new techniques will be discussed.
4.Application of Molecular Biotechnology on the Health (active substances, vaccines, diagnostic
techniques and gene therapy); on the Environment (Toxic elimination and bioremediation); and on the
Agriculture (transgenic plants and animals for the food chain).
5.Laboratory work will be focused on the most common techniques used for the most recent
biotechnological applications
Contents
Theoretical programme
1. Introduction
2. Fundaments of Molecular Cloning and DNA analysis
3. Microbial Systems in Genetic Engineering
4. Eukaryotic Systems in Genetic Engineering
5. Applications of Genetic Engineering for the development of new products and services
6. Official Regulation
Laboratorial programme
1. Training on pipeting. Solutions and dilutions.
2. Plasmidic DNA extraction (MiniPrep)
3. Restriction digestion of plasmidic DNA
4. Agarose gel electrophoresis
5. Preparation of competent bacteria
6. Transformation of competent bacteria
7. Screening of recombinants
8. Autonomous laboratorial work: Identification of unknown DNA sample
1. Introduction
2. Fundaments of Molecular Cloning and DNA analysis
3. Microbial Systems in Genetic Engineering
4. Eukaryotic Systems in Genetic Engineering
5. Applications of Genetic Engineering for the development of new products and services
6. Official Regulation
Laboratorial programme
1. Training on pipeting. Solutions and dilutions.
2. Plasmidic DNA extraction (MiniPrep)
3. Restriction digestion of plasmidic DNA
4. Agarose gel electrophoresis
5. Preparation of competent bacteria
6. Transformation of competent bacteria
7. Screening of recombinants
8. Autonomous laboratorial work: Identification of unknown DNA sample
Teaching Methods
1.Magisterial theoretical exposition of the subjects on study and frequent discussions of actual cases
passed on the media.
2.Execution of laboratorial work programed and predefined by the teacher, following specific written
procedures.
3.Execution of laboratorial work programed and defined by the students as mini-projects, performed under
the supervision of the teacher, for the identification of an unknown DNA
4.Preparation of thematic monographs to be presented as posters at the end of the course by the students.
The themes on discussion are weekly accompanied in the internet.
Evaluation of knowledge is composed of four parts, previously agreed with students:
1.Theoretical component (60%) Written examinations (2 tests for continuous evaluation or final
examination)
2.Practical component (15%) Written Report on the autonomous laboratorial work.
3.Inventive component (15%) Thematic monograph for poster presentation.
4.Assiduity to theoretical and practical sessions (10%)
passed on the media.
2.Execution of laboratorial work programed and predefined by the teacher, following specific written
procedures.
3.Execution of laboratorial work programed and defined by the students as mini-projects, performed under
the supervision of the teacher, for the identification of an unknown DNA
4.Preparation of thematic monographs to be presented as posters at the end of the course by the students.
The themes on discussion are weekly accompanied in the internet.
Evaluation of knowledge is composed of four parts, previously agreed with students:
1.Theoretical component (60%) Written examinations (2 tests for continuous evaluation or final
examination)
2.Practical component (15%) Written Report on the autonomous laboratorial work.
3.Inventive component (15%) Thematic monograph for poster presentation.
4.Assiduity to theoretical and practical sessions (10%)
Teaching Staff
Certificações
