Name: Biochemistry
Code: QUI0348L
Duration: 15 weeks/156 hours
Scientific Area: Biochemistry

Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Regime de Frequência: Presencial

Sustainable Development Goals


I – Lectures Program
1) Introduction to the metabolism. Metabolic pathways and their control. Clinical correlations.
2) Carbohydrate metabolism and their control: glycolysis. gluconeogenesis; glicogenolysis and glycogenesis; pentose phosphate pathways. Piruvic acid degradation.
3)Acetil Co-A pathways. Tricarboxylic acids cycle and their regulation.
4) Electron transport chain and oxidative phosphorylation.
5)Glyoxilic acid cycle. Photosynthesis and photorespiration.
6) Lipid metabolism and their control: Beta-oxidation and bio-synthesis of fatty acids; Ketone bodies; prostaglandins, thromboxanes and leukotrienes; phospholipids; sphingolipids; cholesterol; plasma lipoproteins.
7) Aminoacid metabolism and their control. Purine and pirimidine metabolism. Iron and heme metabolism.
8) Fundamentals of genetic information and expression. Acid nucleic biosynthesis. Transcription. Proteins biosynthesis.
9) Integration of metabolism. Metabolic interrelationships and their control. Role of hormones in Biochemistry.
10) Main metabolic correlations.

II - Laboratorial works:

1- Presentation. The Objectives and avaliationof laboratorial component.
2- Research and administration of Information in Biochemistry
3- Study of phosphate compounds hidrólise
4- Electron transport in thylakoid membrane and proton gradient
5- Oxydative Phosphorilation - Part I
6- Oxydative Phosphorilation - Part II
7- Biomembrane permebilization (Study of metabolic pathways in situ)

Teaching Methods

The teach-learning process will be based in the individual work of the student supported by theoretical and practical classes and by bibliography research. Theoretical classes will be supported by audiovisual techniques and recommended bibliography. In practical classes, students will e distributed in smaller work teams in order to promote student motivation and develop spirit critic, interpretation ability and accuracy. Practical lectures, mainly non laboratorial, will be articulated with theoretical concepts and supported by train some practical examples, discussing subjects and problems solving related with metabolism and metabolic pathways and planned an research work correlated with the contents of this curricular unit.

Recommended Reading

CAMPBELL, M. K., FARRELL, S. O., (2003). Biochemistry, 4th ed., Thomson, USA.
CAMPOS, L. (1987). Guia dos compostos Orgânicos e Bioquímicos – estrutura e nomenclatura. Europress. Colecção Universidades. Odivelas.
CAMPOS, L. (2005). Entender a Bioquímica, 4ª edição. Escolar Editora, Lisboa.
DEVLIN, T.M. (1997). Textbook of Biochemistry with clinical correlations, 4th edition. Wiley-Liss Publication, New York.
FRAUSTO DA SILVA, J. & WILLIAMS, R. (1997). The Biological Chemistry of the Elements. Clarendon Press, Oxford.
Gilbert, H. (1992). Basic Concepts in Biochemistry -a student's survival guide. McGraw-Hill. New York.
HALPERN, M.J. (1997). Bioquímica. Lidel, Edições Técnicas. Lisboa.
HOLME, D.J. & PECK, H. (1998). Analytical Biochemistry, 3rd ed., Longman, United Kingdom.
MATHEWS, C. & HOLDE, K. (1990). Biochemistry. Benjamin-Cummings Publishing Company, Inc., Redwood City.
MCKEE, T & MCKEE, J. (2003). Biochemistry: The molecular Basis of Life, 3nd ed., McGraw-Hill, London.
PLUMMER, D.T. (1987). An introduction to practical Biochemistry, 3rd ed., McGraw Hill, London.
RAWN, J. (1983). Biochemistry. Harper Ed..New York.
ROBYT, J.F.& WHITE, B.J. (1990). Biochemical Tecniques – Theory and Practice, Waveland Press, Inc. United States of America.
STREYER, L. (1995). Biochemistry, 4th ed., WH Freeman and Company, New York.
STRONG, F. & KOCH, G. (1981). Biochemistry Laboratory Manual. 3rd Ed., Wm. C. Brown Publisher. Dubuque.
STRYER, L. (1995). Biochemistry. 4th Ed.. W.H.Freeman and Company. NewYork.
VOET, D., VOET, J. G & PRATT, C.W. (2005). Fundamentals of Biochemistry, 2nd ed., John Wiley & Sons, Inc., New York.
VOET, D. & VOET, J. G (2004). Biochemistry. 3rd ed., John Wiley & Sons, Inc., New York.
ZEIDAN, M. (1996) Experimental Approaches in Biochemistry and Molecular Biology. Wm. C. Brown Publisher.Dubuque.
ZUBAY, G.L., PARSON, W.& VANCE, D (1995). Principles of Biochemistry. Wm. C. Brown Publishers, Dubuke.
ZUBAY, G.L. (1998). Biochemistry. 4th ed. Wm. C. Brown Publishers, Dubuke.

Recommended links:

http://www.gwu.edu/~mpb/ (Metabolic Pathways of Biochemistry)

http://www.bmb.leeds.ac.uk/illingworth/medics1/liver.htm (Hepatic fat & carbohydrate metabolism)