2023
Organic Chemistry applied to Biochemistry
Name: Organic Chemistry applied to Biochemistry
Code: QUI13562L
3 ECTS
Duration: 15 weeks/78 hours
Scientific Area:
Chemistry
Teaching languages: Portuguese
Languages of tutoring support: Portuguese, English
Regime de Frequência: Presencial
Sustainable Development Goals
Learning Goals
This course unit is intended for students to:
- acquire knowledge in the field of organic chemistry applied to biotechnology, in particular as regards the chemistry of secondary metabolites;
- understand and classify the different types of secondary metabolites;
- develop dexterity, ease and safety in laboratory work;
- develop the design and execution skills of laboratory work in the extraction, isolation and identification of secondary metabolites;
- develop critical thinking and problem solving skills.
- acquire knowledge in the field of organic chemistry applied to biotechnology, in particular as regards the chemistry of secondary metabolites;
- understand and classify the different types of secondary metabolites;
- develop dexterity, ease and safety in laboratory work;
- develop the design and execution skills of laboratory work in the extraction, isolation and identification of secondary metabolites;
- develop critical thinking and problem solving skills.
Contents
Secondary metabolites:
- Classification;
- Biological activity.
Derivatization reactions of secondary metabolites.
Separation and isolation techniques of secondary metabolites and their derivatives:
- Chromatography;
- Stationary phases, elution systems and detection modes.
Spectroscopic and spectrometric techniques for structural analysis of organic compounds:
Separation techniques and isolation of organic compounds:
Column chromatography, HPLC and GC.
Stationary phases, eluents and detection methods.
Spectroscopic and spectrometric techniques for structural analysis of organic compounds:
One and two-dimensional of spectrometric techniques of NMR (1H, 13C, DEPT, COSY, HMBC, HMQC, INADEQUATE, NOESY, TOCSY,...).
NMR spectrometry of other important nuclei (15N, 19F, 31P and 29Si).
Infrared spectrometry (FT-IR).
Mass spectrometry.
- Classification;
- Biological activity.
Derivatization reactions of secondary metabolites.
Separation and isolation techniques of secondary metabolites and their derivatives:
- Chromatography;
- Stationary phases, elution systems and detection modes.
Spectroscopic and spectrometric techniques for structural analysis of organic compounds:
Separation techniques and isolation of organic compounds:
Column chromatography, HPLC and GC.
Stationary phases, eluents and detection methods.
Spectroscopic and spectrometric techniques for structural analysis of organic compounds:
One and two-dimensional of spectrometric techniques of NMR (1H, 13C, DEPT, COSY, HMBC, HMQC, INADEQUATE, NOESY, TOCSY,...).
NMR spectrometry of other important nuclei (15N, 19F, 31P and 29Si).
Infrared spectrometry (FT-IR).
Mass spectrometry.
Teaching Methods
The teaching/learning process is based on the students' individual work, supported by the bibliography recommended by the teacher and notes obtained by the students during the classes. The lectures are classes of exposition of the subject during which there is the resolution of some exercises, clarification of doubts and discussion of topics related to the subject taught. The laboratory practical classes work in conjunction with and complement the theoretical and non-laboratory practical classes, using the planning and execution of laboratory work that gives practical examples of the theoretical contents.
The students evaluation in this curricular unit will have two components, a theoretical and a laboratory practice. For the evaluation of the theoretical component students have two optional modalities, the frequency (2 tests) or the final exam.
The final evaluation is given by the contribution of the theoretical components (70%) and laboratory practice (30%).
The students evaluation in this curricular unit will have two components, a theoretical and a laboratory practice. For the evaluation of the theoretical component students have two optional modalities, the frequency (2 tests) or the final exam.
The final evaluation is given by the contribution of the theoretical components (70%) and laboratory practice (30%).
Teaching Staff
- António Manuel Deométrio Rodrigues Lourenço Pereira [responsible]
