2024
Microbiology
Name: Microbiology
Code: BIO00408L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biological Sciences
Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Presentation
The importance of microbes and their study in multiple human activities and on environmental balances is highlighted. Microbes as drivers of developed and sustainable societies.
Sustainable Development Goals
Learning Goals
Study of microbes with emphasis on the relationships with different habitats, its influence on man's current activities and contribution to the new achievements of biological knowledge at the molecular level.
A - Identify and recognize the major microbial groups, Bacteria, Archaea, Fungi, Protists and Viruses.
B - Know the risks and methodologies associated with the handling of microbes, Asepsis, Pure Cultures, Shape and Measurement of Microbial Growth.
C - Identify the control methods of microbial populations
D - Recognize the microbial metabolism and its impact on the environment including use by man of the resulting products.
E - Know the basics of molecular microbiology, Microbial Genetics, Immunology and Virology.
F - Recognize the importance of microorganisms in food processing and conservation and associated risks.
G - Identify the role of microorganisms and their associations on health, biogeochemical cycles, agriculture and other human activities.
A - Identify and recognize the major microbial groups, Bacteria, Archaea, Fungi, Protists and Viruses.
B - Know the risks and methodologies associated with the handling of microbes, Asepsis, Pure Cultures, Shape and Measurement of Microbial Growth.
C - Identify the control methods of microbial populations
D - Recognize the microbial metabolism and its impact on the environment including use by man of the resulting products.
E - Know the basics of molecular microbiology, Microbial Genetics, Immunology and Virology.
F - Recognize the importance of microorganisms in food processing and conservation and associated risks.
G - Identify the role of microorganisms and their associations on health, biogeochemical cycles, agriculture and other human activities.
Contents
Theoretical:
1. Historical context and Ubiquity
2. Diversity of the Microbial World
3. Microbial Growth and Death
4. Metabolism
5. Basics of Molecular Microbiology: Microbial genetics, Virology, Immunology
6. Microbes and disease; Normal flora, Pathology, infection and disease, Mechanisms of pathogenicity, Principles of epidemiology
7. Food microbiology: Hygiene and concept of indicator. Processing and storage of food. Foodborne diseases
8. Ecology and environmental microbiology: Soil and water, Biogeochemical cycles, Agricultural applications, Wastewater treatment, Biotechnology applications
Lab Practice:
Aseptic practice
Observation of bacteria, fungi and protists.
Demonstration of Ubiquity
Preparation and sterilization of culture media.
Isolation of pure culture.
Colonial and cellular morphology. Staining methods
Microbial counts
Environmental conditions for growth (pH, temp., O2)
Anaerobic Culture
Antibiograms
Microbial spreading simulation
Water and milk analises
Plant symbiosis.
1. Historical context and Ubiquity
2. Diversity of the Microbial World
3. Microbial Growth and Death
4. Metabolism
5. Basics of Molecular Microbiology: Microbial genetics, Virology, Immunology
6. Microbes and disease; Normal flora, Pathology, infection and disease, Mechanisms of pathogenicity, Principles of epidemiology
7. Food microbiology: Hygiene and concept of indicator. Processing and storage of food. Foodborne diseases
8. Ecology and environmental microbiology: Soil and water, Biogeochemical cycles, Agricultural applications, Wastewater treatment, Biotechnology applications
Lab Practice:
Aseptic practice
Observation of bacteria, fungi and protists.
Demonstration of Ubiquity
Preparation and sterilization of culture media.
Isolation of pure culture.
Colonial and cellular morphology. Staining methods
Microbial counts
Environmental conditions for growth (pH, temp., O2)
Anaerobic Culture
Antibiograms
Microbial spreading simulation
Water and milk analises
Plant symbiosis.
Teaching Methods
In theoretical classroom sessions (2 h) the content of the UC program is presented, seeking to encourage the discussion of aspects related to microbes impact in daily life of human societies. The theoretical concepts are supported by slides, available on the Moodle platform. The evaluation of theoretical (T) is held on two frequencies or a final exam.
The practical component (P) is carried out on laboratory sessions (2 h), where each proposed theme is developed experimentally by executing protocols taken from support manual, available in Moodle.
The laboratory work is performed by groups of 3 students. At the end of the semester there will be a practical test (TP).
The final grade of the UC is obtained by the expression: CF=0.70*T+0.30*P.
The practical component (P) is carried out on laboratory sessions (2 h), where each proposed theme is developed experimentally by executing protocols taken from support manual, available in Moodle.
The laboratory work is performed by groups of 3 students. At the end of the semester there will be a practical test (TP).
The final grade of the UC is obtained by the expression: CF=0.70*T+0.30*P.
Teaching Staff (2023/2024 )
