2023

Biochemical Analysis II

Name: Biochemical Analysis II
Code: QUI12395L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area: Biochemistry

Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Regime de Frequência: Presencial

Presentation

Course unit focused on teaching / learning of various modern analytical methodologies of preparation and analysis of biological samples, and of materials with strong biochemical impact.

Sustainable Development Goals

Learning Goals

Through this curricular unit it is intended that students acquire and expand their knowledge, skills and competences on fundamental and modern methods of preparation, treatment, characterization and physicochemical analysis of samples in the context of biochemical analysis and that, in a solid and coherent way, they are able to select and apply them judiciously and appropriately to real cases.

Contents

Fundamentals, instrumentation, practical aspects and applications in Biochemistry of:
i) Vis/UV atomic spectrometry, with particular emphasis on the introduction to inductively coupled plasma spectroscopies (ICP-AES/OES and ICP-MS);
ii) Raman spectroscopies;
iii) Voltammetric and amperometric electrochemical methods;
iv) Electrochemical Biosensors (including the immunosensors);
v) Mass spectrometry and hyphenation to chromatographic analysis methods (GC-MS e LC-MS);
vi) Immunochemistry, production and analytical application of specific antibodies.
vii) Radiochemistry: Equipment, detection of radioactivity by liquid scintillation, applications. Intelligent data analysis: Data Mining applied to biochemistry databases; Treatment and quality of information; Trust score.
Mono and 2D NMR for structural analysis of biomolecules (1H, 13C, DEPT, COSY, HMBC, HMQC, INADEQUATE, NOESY, TOCSY,…); NMR of other important nuclei (15N, 19F, 31P e 29Si).

Teaching Methods

The teaching / learning process will be based on the student's work, through the contents taught and the guidance provided by the teacher, and will include moments of consultation and research. To promote the active and critical participation of students will be used didactic resources and dialectical teaching methods. Theoretical contents will be introduced in non-laboratory practical classes and consolidated through laboratory practical classes (PL), after which a work / report / questionnaire can be requested for evaluation. In most PL classes students will have contact with research work and advanced modern equipment. The knowledge assessment will be based on a continuous assessment system, CA (ratings of three Frequencies, F, and of a written work, W), or a final assessment system, FA (ratings of a final exam, FE, and above-mentioned work). Weighting: AC - F1 (29.79%) + F2 (39.72%) + F3 (19.88%) + W (10.61%); FA - FE (89.39%) + W (10.61%).