2024
Enzymology
Name: Enzymology
Code: QUI12396L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biochemistry
Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Presentation
Purification, structural and functional characterization of enzymes. Theoretical and experimental models. Applications in medicine, pharmacology, agronomy and environmental control.
Sustainable Development Goals
Learning Goals
1. To know and to understand theoretical and experimental information of enzymology that includes terms, concepts, factors, principles, laws, theories, methods and procedures that make it possible to study the enzymes and its interaction with surrounding environment.
2. Develop a critical aptitude to equate new problems in domain of enzymology which allow a conception of experimental strategies to clarify the biological role of enzymes and its eventual application in biomedicine or biotechnology.
3. To apply the acquired knowledge to new situations in domain of enzymology, relatively to utilization of concepts, laws, methods, ethic and deontological aspects of biochemistry.
4. To execute, in accurate form, experimental procedures to characterize structural and functional aspects of enzymes.
5. To develop capacity to organize, in group and using informatics bases, work laboratorial planes to improve the knowledge about enzymes and its biomedical or biotechnological applications, useful for prepare scientific communications and laboratorial management.
2. Develop a critical aptitude to equate new problems in domain of enzymology which allow a conception of experimental strategies to clarify the biological role of enzymes and its eventual application in biomedicine or biotechnology.
3. To apply the acquired knowledge to new situations in domain of enzymology, relatively to utilization of concepts, laws, methods, ethic and deontological aspects of biochemistry.
4. To execute, in accurate form, experimental procedures to characterize structural and functional aspects of enzymes.
5. To develop capacity to organize, in group and using informatics bases, work laboratorial planes to improve the knowledge about enzymes and its biomedical or biotechnological applications, useful for prepare scientific communications and laboratorial management.
Contents
1. Catalitic activity of proteins and RNA. Terminology. Reaction curves, deviation to linearity, v0, Eact, transition state complex.
2. Continuous and descontinuous assays to v0 determination. Burst and lag phases. Interferences in v0 determination
3. The Henri-Michaelis-Menten equation. Parameters Vmax e Km. Effects of [E], T, pH, [I] [A], [S]. Failure to obey rectangular hyperbola, km and Vmax determination, differents graphics models.
4. Units and specific activity.
5. Mechanisms of enzymatic reaction, reactions of more than one substrate, enzymatic inhibition.
6. Regulation of enzymatic activity. Post-translation modifications. Alostery and cooperativity, mathematics models.
7. Extraction, solubilization and purification of enzymes, homogenization, centrifugation, organics solvents, polymers e chromatographic methods. 8. Physico-chemical characterization of enzymes.
Teaching Methods
1. Lectures are plenary lessons structured in the scientific method which combine subject exposition and bibliographic-assisted search for generating capacities to understand strategies and to apply methodologies which allow clarifying chemistry properties, reaction mechanisms and regulation mechanisms of enzymes as well as its application.
2. Practical classes take place in teaching laboratories where the students execute laboratory-assisted work following protocols previously supplied by the teacher or delineated by the student resorting to bibliographic information. Also will teach instructions to write a report.
3. Tutorial classes involve instants of individual contacts or in little teams where search, read and assisted-interpretation of scientific papers will contribute to preparing experimental assays and the project. Evaluation: Theoretical - continuous (30%) project (monograph/discussion) (30%); experimental component (40%).
2. Practical classes take place in teaching laboratories where the students execute laboratory-assisted work following protocols previously supplied by the teacher or delineated by the student resorting to bibliographic information. Also will teach instructions to write a report.
3. Tutorial classes involve instants of individual contacts or in little teams where search, read and assisted-interpretation of scientific papers will contribute to preparing experimental assays and the project. Evaluation: Theoretical - continuous (30%) project (monograph/discussion) (30%); experimental component (40%).
Teaching Staff (2023/2024 )
