2024
Enzyme Technology
Name: Enzyme Technology
Code: QUI13614L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biochemistry
Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Regime de Frequência: Presencial
Sustainable Development Goals
Learning Goals
The aim of this program course is to provide the students with Knowledge on enzymes technology to understand genetic concepts and industrial application on the enzymatic field. Students will be able to Know and understanding the main biotechnological concepts applied to the enzyme production and protein engineering technologies. Students will be able to execute laboratorial projects, solving theoretical problems applied to the isolation, purification and characterization of enzymes produced by microorganisms and apply the acquired knowledge to a new situations correlated to Biochemistry, Biotechnology and Medicine.
This curricular unit also intends to develop a set of skills to collect, select and interpret relevant scientific information, and communicate ideas and scientific knowledge, orally and written, organized in a coherent and logical form, about matters within the scope of the unit.
This curricular unit also intends to develop a set of skills to collect, select and interpret relevant scientific information, and communicate ideas and scientific knowledge, orally and written, organized in a coherent and logical form, about matters within the scope of the unit.
Contents
Enzymes properties and kinetics (revision).
Enzyme production: Sources of enzymes; advantages and disadvantages of enzyme production and extraction from microbial strains, plants and animals. Factors affecting enzyme production from microbial sources. Optimization of enzyme production. Enzyme production by fermentation. Submerged and solid state fermentations. Extraction and purification of enzymes. Downstream processing. Removal of cells, purification and final isolation. Chromatographic techniques: Affinity, immunoaffinity, ion-exchange, hydrophobic interaction, gel filtration and immobilized metal affinity chromatography (IMAC).
Chemical modification of proteins. Protein engineering: site-directed mutagenesis of enzyme gene and overproduction of transformed enzymes.
Immobilization of biocatalysts. Methods of immobilization. Advantages and disadvantages of immobilized enzymes and cells.
Reactors for immobilized and free biocatalysts. Industrial applications of biocatalysts.
Enzyme production: Sources of enzymes; advantages and disadvantages of enzyme production and extraction from microbial strains, plants and animals. Factors affecting enzyme production from microbial sources. Optimization of enzyme production. Enzyme production by fermentation. Submerged and solid state fermentations. Extraction and purification of enzymes. Downstream processing. Removal of cells, purification and final isolation. Chromatographic techniques: Affinity, immunoaffinity, ion-exchange, hydrophobic interaction, gel filtration and immobilized metal affinity chromatography (IMAC).
Chemical modification of proteins. Protein engineering: site-directed mutagenesis of enzyme gene and overproduction of transformed enzymes.
Immobilization of biocatalysts. Methods of immobilization. Advantages and disadvantages of immobilized enzymes and cells.
Reactors for immobilized and free biocatalysts. Industrial applications of biocatalysts.
Teaching Methods
The teaching is based on theoretical classes, laboratory practice, and tutorial guidance. Laboratory practice is articulated with the theoretical component, applying the content covered to concrete situations. Tutorial guidance provides scientific and pedagogical support and follow-up to students, particularly in carrying out an integrative complementary project. The approach to the contents of this course unit is based on methodologies that combine the structured transmission of knowledge with the active participation of students, promoting the articulation between concepts and their practical application in different contexts. Content is presented in a systematic manner and supported by audiovisual resources, enabling the explanation of fundamental concepts illustrated with examples that facilitate understanding. In parallel, students are engaged in activities of observation, analysis, and problem-solving, encouraging critical interpretation, discussion, and the exchange of perspectives.
The methodology also includes the development of a semester-long project, which challenges students to apply the knowledge acquired to the resolution of a specific situation or problem. This work involves the planning, design, and implementation of different stages, reinforcing organizational skills, knowledge integration, autonomy, and collaboration.
This student-centered pedagogical model ensures the consolidation of knowledge and the development of transversal skills, contributing to the scientific, technical, and interpersonal training required by the course unit profile.
The methodology also includes the development of a semester-long project, which challenges students to apply the knowledge acquired to the resolution of a specific situation or problem. This work involves the planning, design, and implementation of different stages, reinforcing organizational skills, knowledge integration, autonomy, and collaboration.
This student-centered pedagogical model ensures the consolidation of knowledge and the development of transversal skills, contributing to the scientific, technical, and interpersonal training required by the course unit profile.
Assessment
Continuous Assessment
? Two written tests carried out during the semester, covering the content of the theoretical component, with a minimum grade of 8 (out of 20) in each test and a minimum average grade of 10 (out of 20) (60%).
? Performance in laboratory classes, preparation of reports and their oral discussion, and oral presentation of a project in based on selected scientific articles (40%).
Final Examination
? Final written exam, with a minimum passing grade of 10 (out of 20) (60%).
? Practical component grade (40%), based on performance in laboratory classes, preparation of reports, and oral discussion of the work.
Working students who choose not to attend laboratory classes, or students who do not achieve a pass grade in the laboratory component, may be evaluated through the final examination. In this case, to obtain the practical component grade, it will be necessary to submit an individual report or take an exam on the laboratory practice component.
? Two written tests carried out during the semester, covering the content of the theoretical component, with a minimum grade of 8 (out of 20) in each test and a minimum average grade of 10 (out of 20) (60%).
? Performance in laboratory classes, preparation of reports and their oral discussion, and oral presentation of a project in based on selected scientific articles (40%).
Final Examination
? Final written exam, with a minimum passing grade of 10 (out of 20) (60%).
? Practical component grade (40%), based on performance in laboratory classes, preparation of reports, and oral discussion of the work.
Working students who choose not to attend laboratory classes, or students who do not achieve a pass grade in the laboratory component, may be evaluated through the final examination. In this case, to obtain the practical component grade, it will be necessary to submit an individual report or take an exam on the laboratory practice component.
Teaching Staff
- Ana Paula Honrado Pinto
- Ana Teresa Fialho Caeiro Caldeira [responsible]
Certificações
