2023
Methods and Techniques in Biomedicine
Name: Methods and Techniques in Biomedicine
Code: CMS14304L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biomedical Sciences
Teaching languages: Portuguese
Languages of tutoring support: Portuguese, English
Regime de Frequência: Presencial
Presentation
This UC provides a comprehensive knowledge in the methodologies in the field of the Biomedical sciences and Biomedicine.
Sustainable Development Goals
Learning Goals
The general objective of this course is to provide students with a set of knowledge of techniques and methodologies that allow them to perform adequately in the laboratory of Biomedicine, Biomedical Sciences and Life Sciences in general.
Specifically, it is intended that students have techniques and methods available for the detection and analysis of biomolecules, cells and tissues and that they acquire technical and methodological knowledge for the study of scientific problems in the aforementioned areas.
Students should also be able to apply the acquired knowledge to new and concrete situations.
Specifically, it is intended that students have techniques and methods available for the detection and analysis of biomolecules, cells and tissues and that they acquire technical and methodological knowledge for the study of scientific problems in the aforementioned areas.
Students should also be able to apply the acquired knowledge to new and concrete situations.
Contents
Molecular absorption spectrometry, fluorescence and fluorimetry.
Cell fractionation: purification and identification of biomolecules.
Detection and analysis of proteins and lipids (electrophoresis; chromatography; Staining and quantification methods; identification; structural analysis).
Detection and analysis of nucleic acids: Basic methods; DNA sequencing; Detection and quantitation of RNA.
Immunological techniques: Monoclonal antibodies; Purification and use of antibodies.
Cell isolation and cell culture: Flow cytometry and fluorescence activated cell sorting.
Microscopy - imaging of biological specimens: Light microscopy; Fluorescence and laser confocal microarray microscopy; Electron Microscopy (EM); Magnetic resonance imaging (MRI).
The derivation and manipulation of experimental animals in biomedical sciences.
Electrochemical techniques: voltametry, amperometry and their applications in biomedicine.
Radioisotopes and their applications.
Electrophysiological methods.
Cell fractionation: purification and identification of biomolecules.
Detection and analysis of proteins and lipids (electrophoresis; chromatography; Staining and quantification methods; identification; structural analysis).
Detection and analysis of nucleic acids: Basic methods; DNA sequencing; Detection and quantitation of RNA.
Immunological techniques: Monoclonal antibodies; Purification and use of antibodies.
Cell isolation and cell culture: Flow cytometry and fluorescence activated cell sorting.
Microscopy - imaging of biological specimens: Light microscopy; Fluorescence and laser confocal microarray microscopy; Electron Microscopy (EM); Magnetic resonance imaging (MRI).
The derivation and manipulation of experimental animals in biomedical sciences.
Electrochemical techniques: voltametry, amperometry and their applications in biomedicine.
Radioisotopes and their applications.
Electrophysiological methods.
Teaching Methods
The teaching/learning process is based on the individual work of the students, supported by theoretical practices (26h) and laboratory practices (39h) and the recommended bibliography.
The evaluation system is organized to consider the work carried out by the student in the different components. The practical component (PS) is continuously evaluated considering the attendance, the performance in the laboratory and the analysis, interpretation and discussion of results (oral and/or essay). The theoretical-practical component (TS) can be evaluated in two modalities: continuous (written test) or by final exam.
The final score (FS) will be calculated on base of practical component score (PS), and of the theoretical component score (FS): FS=0.25*PS+0.75*TS.
The evaluation system is organized to consider the work carried out by the student in the different components. The practical component (PS) is continuously evaluated considering the attendance, the performance in the laboratory and the analysis, interpretation and discussion of results (oral and/or essay). The theoretical-practical component (TS) can be evaluated in two modalities: continuous (written test) or by final exam.
The final score (FS) will be calculated on base of practical component score (PS), and of the theoretical component score (FS): FS=0.25*PS+0.75*TS.
