2025
Medical Microbiology II
Name: Medical Microbiology II
Code: MVT15200I
3 ECTS
Duration: 15 weeks/78 hours
Scientific Area:
Veterinary Medicine
Teaching languages: Portuguese
Languages of tutoring support: Portuguese, English
Regime de Frequência: Presencial
Sustainable Development Goals
Learning Goals
The learning objectives are:
• To know the aspects of the biology and pathogenesis of viruses, in order to establish the most appropriate control strategies.
• To know the various types of viruses with Veterinary importance.
The skills to be developed are:
• Ability to manipulate laboratory instrumentation and the various techniques in the treatment of clinical samples containing viruses;
• Ability to plan and perform virological tests;
• Ability to produce technical reports.
• To know the aspects of the biology and pathogenesis of viruses, in order to establish the most appropriate control strategies.
• To know the various types of viruses with Veterinary importance.
The skills to be developed are:
• Ability to manipulate laboratory instrumentation and the various techniques in the treatment of clinical samples containing viruses;
• Ability to plan and perform virological tests;
• Ability to produce technical reports.
Contents
Theoretical
1. Origin of viruses. Virology as a science.
2. General properties of viruses.
3. Genome and genomic organization. Structural and non-structural proteins.
4. Taxonomy.
5. Replicative cycle. Viruses with DNA genome. Viruses with RNA genome.
6. Exit routes of host cell.
7. Synchronized replication cycle - one-step replication cycle.
8. Viral genetics: Recombination and reassortment.
9. Viral oncogenesis.
10. Viral pathogenesis: Interactions between host and virus.
11. Immune response to viral infections.
12. Virulence. Streaming. Epidemiology. Types of infection.
Practical
Molecular methods of virus detection and identification.
Inoculation of eggs.
Rapid hemagglutination.
Titration of hemagglutinating viruses by the micromethod.
Inhibition of hemagglutination (HI).
Cell culture.
Titration of viruses in cell culture.
Cytopathic effect and determination of TCID50.
Formation of lysis plates and determination of 100 PFU.
Viral neutralization and seroneutralization.
1. Origin of viruses. Virology as a science.
2. General properties of viruses.
3. Genome and genomic organization. Structural and non-structural proteins.
4. Taxonomy.
5. Replicative cycle. Viruses with DNA genome. Viruses with RNA genome.
6. Exit routes of host cell.
7. Synchronized replication cycle - one-step replication cycle.
8. Viral genetics: Recombination and reassortment.
9. Viral oncogenesis.
10. Viral pathogenesis: Interactions between host and virus.
11. Immune response to viral infections.
12. Virulence. Streaming. Epidemiology. Types of infection.
Practical
Molecular methods of virus detection and identification.
Inoculation of eggs.
Rapid hemagglutination.
Titration of hemagglutinating viruses by the micromethod.
Inhibition of hemagglutination (HI).
Cell culture.
Titration of viruses in cell culture.
Cytopathic effect and determination of TCID50.
Formation of lysis plates and determination of 100 PFU.
Viral neutralization and seroneutralization.
Teaching Methods
This subject will be taught over half the semester (7 weeks).
Each week will consist of a theoretical lecture (120 minutes) and a practical laboratory lecture (120 minutes).
The theoretical lectures will be plenary sessions dedicated to acquiring basic concepts and knowledge.
The practical lectures will consist of practical assignments developed by the students after a demonstration by the professor. This hands-on work will promote the acquisition of the desired skills.
Each week will consist of a theoretical lecture (120 minutes) and a practical laboratory lecture (120 minutes).
The theoretical lectures will be plenary sessions dedicated to acquiring basic concepts and knowledge.
The practical lectures will consist of practical assignments developed by the students after a demonstration by the professor. This hands-on work will promote the acquisition of the desired skills.
Assessment
Continuous assessment consists of a written theoretical test (50%) and an oral practical test (50%). Each test has a minimum grade of 10 or above.
The final assessment consists of a written test and an individual oral practical exam. Each test has a minimum passing grade of 10 points.
Students who have not attended at least 75% of the practical classes will have to take an extra oral practical exam and obtain a minimum grade of 10 points. The practical grade will be the arithmetic mean of these two grades.
The grade results from 50% of the theoretical and 50% of the practical.
In the Special Period and Extraordinary Period, the exam is oral, including both theoretical and practical material.
The final assessment consists of a written test and an individual oral practical exam. Each test has a minimum passing grade of 10 points.
Students who have not attended at least 75% of the practical classes will have to take an extra oral practical exam and obtain a minimum grade of 10 points. The practical grade will be the arithmetic mean of these two grades.
The grade results from 50% of the theoretical and 50% of the practical.
In the Special Period and Extraordinary Period, the exam is oral, including both theoretical and practical material.
Teaching Staff
Accreditations and Certifications
