2025
Genetic Engineering and Pharmaceutical Biotechnology
Name: Genetic Engineering and Pharmaceutical Biotechnology
Code: CMS13778I
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biochemistry, Biological Sciences
Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Regime de Frequência: Presencial
Presentation
Study of the concepts, processes, methodologies and strategies involved in the use of molecular knowledge and its application for recombinant DNA technologies.
Sustainable Development Goals
Learning Goals
1. Understanding Genetic Engineering as an integrated science from several other scientific areas, with focus on a Biologic point of view
2. The teaching will be directed for the familiarization of techniques for the creation of added value from new processes and products.
3. Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology, concepts for application of the new techniques will be discussed.
4. Application of Molecular Biotechnology on the Health (active substances, vaccines, diagnostic techniques and gene therapy); on the Environment (Toxic elimination and bioremediation); and on the Agriculture (transgenic plants and animals for the food chain).
5. Laboratory work will be focused on the most common techniques used for the most recent biotechnological applications.
2. The teaching will be directed for the familiarization of techniques for the creation of added value from new processes and products.
3. Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology, concepts for application of the new techniques will be discussed.
4. Application of Molecular Biotechnology on the Health (active substances, vaccines, diagnostic techniques and gene therapy); on the Environment (Toxic elimination and bioremediation); and on the Agriculture (transgenic plants and animals for the food chain).
5. Laboratory work will be focused on the most common techniques used for the most recent biotechnological applications.
Contents
Theoretical programme
1. Introduction
2. Fundaments of Molecular Cloning and DNA analysis
3. Microbial Systems in Genetic Engineering
4. Eukaryotic Systems in Genetic Engineering
5. Applications of Genetic Engineering for the development of new products and services
6. Official Regulation
Laboratorial programme
1. Training on pipeting. Solutions and dilutions.
2. Plasmidic DNA extraction (MiniPrep)
3. Restriction digestion of plasmidic DNA
4. Agarose gel electrophoresis
5. Preparation of competent bacteria
6. Transformation of competent bacteria
7. Screening of recombinants
8. Autonomous laboratorial work: Identification of unknown DNA sample
1. Introduction
2. Fundaments of Molecular Cloning and DNA analysis
3. Microbial Systems in Genetic Engineering
4. Eukaryotic Systems in Genetic Engineering
5. Applications of Genetic Engineering for the development of new products and services
6. Official Regulation
Laboratorial programme
1. Training on pipeting. Solutions and dilutions.
2. Plasmidic DNA extraction (MiniPrep)
3. Restriction digestion of plasmidic DNA
4. Agarose gel electrophoresis
5. Preparation of competent bacteria
6. Transformation of competent bacteria
7. Screening of recombinants
8. Autonomous laboratorial work: Identification of unknown DNA sample
Teaching Methods
1. Theoretical presentations
2. Bench working according with predefined technical protocols
3. Oriented free laboratory work
4. Monograph presentations as public posters
2. Bench working according with predefined technical protocols
3. Oriented free laboratory work
4. Monograph presentations as public posters
Assessment
The final mark may be based on one of the following assessment methods: continuous assessment or final examination.
Continuous assessment will include:
Assessment of the practical-laboratory component, which will focus on the evaluation of practical-laboratory knowledge and the final project for the course (30%).
Assessment of the theoretical-practical component, which will focus on the evaluation of knowledge and its application (10%)
Assessment of the theoretical component will be carried out through two written assessments (60%). The minimum mark required to sit the second assessment must be 9.5 or above, and the average of the two assessments must be 9.5 or above to pass the course unit.
The final assessment will be conducted via a final written examination, for which a mark of 9.5 or above is required to pass the module (100%).
Continuous assessment will include:
Assessment of the practical-laboratory component, which will focus on the evaluation of practical-laboratory knowledge and the final project for the course (30%).
Assessment of the theoretical-practical component, which will focus on the evaluation of knowledge and its application (10%)
Assessment of the theoretical component will be carried out through two written assessments (60%). The minimum mark required to sit the second assessment must be 9.5 or above, and the average of the two assessments must be 9.5 or above to pass the course unit.
The final assessment will be conducted via a final written examination, for which a mark of 9.5 or above is required to pass the module (100%).
Teaching Staff
- Bárbara Raquel Dias Advinha
- Cátia Moreira de Sousa [responsible]
Accreditations and Certifications
