2024
Biotechnology
Name: Biotechnology
Code: BIO12356L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biological Sciences
Teaching languages: Portuguese
Languages of tutoring support: Portuguese, English
Regime de Frequência: Presencial
Sustainable Development Goals
Learning Goals
1. Understanding Genetic Engineering as an integrated science from several other scientific areas, with
focus on a Biologic point of view
2. The teaching will be directed for the familiarization of techniques for the creation of added value from
new processes and products.
3. Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology,
concepts for application of the new techniques will be discussed.
4. Application of Molecular Biotechnology on the Health); on the Environment; and on the Agriculture
(transgenic plants and animals for the food chain).
5. Laboratory work will be focused on the most common techniques used for the most recent
biotechnological applications.
focus on a Biologic point of view
2. The teaching will be directed for the familiarization of techniques for the creation of added value from
new processes and products.
3. Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology,
concepts for application of the new techniques will be discussed.
4. Application of Molecular Biotechnology on the Health); on the Environment; and on the Agriculture
(transgenic plants and animals for the food chain).
5. Laboratory work will be focused on the most common techniques used for the most recent
biotechnological applications.
Contents
1 Theoretical programme
a. Introduction
b. Fundaments of Molecular Cloning and DNA analysis
c. Microbial Systems in Genetic Engineering
d. Eukaryotic Systems in Genetic Engineering
e. Applications of Genetic Engineering for the development of new products and services
f. Official Regulation
2. Laboratorial programme
a. Training on pipeting. Solutions and dilutions.
b. Plasmidic DNA extraction (MiniPrep)
c. Restriction digestion of plasmidic DNA
d. Agarose gel electrophoresis
e. Preparation of competent bacteria
f. Transformation of competent bacteria
g. Screening of recombinants
h. Autonomous laboratorial work: Identification of unknown DNA sample
a. Introduction
b. Fundaments of Molecular Cloning and DNA analysis
c. Microbial Systems in Genetic Engineering
d. Eukaryotic Systems in Genetic Engineering
e. Applications of Genetic Engineering for the development of new products and services
f. Official Regulation
2. Laboratorial programme
a. Training on pipeting. Solutions and dilutions.
b. Plasmidic DNA extraction (MiniPrep)
c. Restriction digestion of plasmidic DNA
d. Agarose gel electrophoresis
e. Preparation of competent bacteria
f. Transformation of competent bacteria
g. Screening of recombinants
h. Autonomous laboratorial work: Identification of unknown DNA sample
Teaching Methods
1 Theoretical presentations
. Bench working according with predefined technical protocols
. Oriented free laboratory work
. Monograph presentations as public posters
. Bench working according with predefined technical protocols
. Oriented free laboratory work
. Monograph presentations as public posters
Certificações
