2024
Genetic Engineering and Pharmaceutical Biotechnology
Name: Genetic Engineering and Pharmaceutical Biotechnology
Code: CMS13778I
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area:
Biochemistry, Biological Sciences
Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Presentation
Study of the concepts, processes, methodologies and strategies involved in the use of molecular knowledge and its application for recombinant DNA technologies.
Sustainable Development Goals
Learning Goals
1. Understanding Genetic Engineering as an integrated science from several other scientific areas, with focus on a Biologic point of view
2. The teaching will be directed for the familiarization of techniques for the creation of added value from new processes and products.
3. Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology, concepts for application of the new techniques will be discussed.
4. Application of Molecular Biotechnology on the Health (active substances, vaccines, diagnostic techniques and gene therapy); on the Environment (Toxic elimination and bioremediation); and on the Agriculture (transgenic plants and animals for the food chain).
5. Laboratory work will be focused on the most common techniques used for the most recent biotechnological applications.
2. The teaching will be directed for the familiarization of techniques for the creation of added value from new processes and products.
3. Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology, concepts for application of the new techniques will be discussed.
4. Application of Molecular Biotechnology on the Health (active substances, vaccines, diagnostic techniques and gene therapy); on the Environment (Toxic elimination and bioremediation); and on the Agriculture (transgenic plants and animals for the food chain).
5. Laboratory work will be focused on the most common techniques used for the most recent biotechnological applications.
Contents
Theoretical programme
1. Introduction
2. Fundaments of Molecular Cloning and DNA analysis
3. Microbial Systems in Genetic Engineering
4. Eukaryotic Systems in Genetic Engineering
5. Applications of Genetic Engineering for the development of new products and services
6. Official Regulation
Laboratorial programme
1. Training on pipeting. Solutions and dilutions.
2. Plasmidic DNA extraction (MiniPrep)
3. Restriction digestion of plasmidic DNA
4. Agarose gel electrophoresis
5. Preparation of competent bacteria
6. Transformation of competent bacteria
7. Screening of recombinants
8. Autonomous laboratorial work: Identification of unknown DNA sample
1. Introduction
2. Fundaments of Molecular Cloning and DNA analysis
3. Microbial Systems in Genetic Engineering
4. Eukaryotic Systems in Genetic Engineering
5. Applications of Genetic Engineering for the development of new products and services
6. Official Regulation
Laboratorial programme
1. Training on pipeting. Solutions and dilutions.
2. Plasmidic DNA extraction (MiniPrep)
3. Restriction digestion of plasmidic DNA
4. Agarose gel electrophoresis
5. Preparation of competent bacteria
6. Transformation of competent bacteria
7. Screening of recombinants
8. Autonomous laboratorial work: Identification of unknown DNA sample
Teaching Methods
1. Theoretical presentations
2. Bench working according with predefined technical protocols
3. Oriented free laboratory work
4. Monograph presentations as public posters
The evaluation of the theoretical component, by continuous assessment of frequencies or final examination, will have a relative weight of 70%.
The practical component will be evaluated by the report of the work performed; it will have a relative weight of 15%.
The evaluation of the monograph and its public presentation will have a relative weight of 15%.
If and when it is possible to do all teaching in the classroom, the relative weight of the monograph and the practical component will be reduced to 10% and 10% will be attributed to the attendance of students to all the components.
2. Bench working according with predefined technical protocols
3. Oriented free laboratory work
4. Monograph presentations as public posters
The evaluation of the theoretical component, by continuous assessment of frequencies or final examination, will have a relative weight of 70%.
The practical component will be evaluated by the report of the work performed; it will have a relative weight of 15%.
The evaluation of the monograph and its public presentation will have a relative weight of 15%.
If and when it is possible to do all teaching in the classroom, the relative weight of the monograph and the practical component will be reduced to 10% and 10% will be attributed to the attendance of students to all the components.
Certificações
