2024
Clinical Laboratory Virology
Name: Clinical Laboratory Virology
Code: CMS13768I
3 ECTS
Duration: 15 weeks/78 hours
Scientific Area:
Ciências Farmacêuticas
Teaching languages: Portuguese
Languages of tutoring support: Portuguese
Presentation
This UC presents the biological and molecular bases that make possible to understand the functioning and justify the existence of viruses, addressing aspects of their taxonomy and systematics, and the technologies, tools and practical procedures for laboratory manipulation of prokaryotic viruses.
Sustainable Development Goals
Learning Goals
1. The main goal for the discipline is to understand the molecular biology of sub-microscopic organisms in order to facilitate the comprehension of the world and its changes. Several examples of mediatic value will be used (AIDS. FLU H5N1, BSE)
2. In the theoretical ground the biological and molecular bases to understand virus will be discussed, with some incursions into the taxonomic aspects of the most relevant virus.
3. Using E. coli bacteriophages, low hazard level material, the technologies for the study of unseen organisms will be developed and discussed.
2. In the theoretical ground the biological and molecular bases to understand virus will be discussed, with some incursions into the taxonomic aspects of the most relevant virus.
3. Using E. coli bacteriophages, low hazard level material, the technologies for the study of unseen organisms will be developed and discussed.
Contents
Theoretical Programme
1. Introduction and functioning of the course
2. General and Molecular Virology
3. Taxonomy and Sistematics
4. Infection and infectious agents
5. Immunology of viral infections
6. Epidemiology of viral diseases
7. Treatment and prevention of viral diseases
8. Diagnostic of viruses
9. Biotechnological applications of virus
Laboratory Programme
1. Theoretical introduction. Biosafety in the laboratory.
2. Experimental study of virus
3. Plant viral Infection (tobacco)
4. Bacterial growth curve
5. Preparation of an elevated titre virus
6. Dosing of virus Plaque forming assay
7. Dosing of virus Limiting dilutions
Autonomous laboratory work: isolation and characterisation of a wild bacteriophage.
1. Introduction and functioning of the course
2. General and Molecular Virology
3. Taxonomy and Sistematics
4. Infection and infectious agents
5. Immunology of viral infections
6. Epidemiology of viral diseases
7. Treatment and prevention of viral diseases
8. Diagnostic of viruses
9. Biotechnological applications of virus
Laboratory Programme
1. Theoretical introduction. Biosafety in the laboratory.
2. Experimental study of virus
3. Plant viral Infection (tobacco)
4. Bacterial growth curve
5. Preparation of an elevated titre virus
6. Dosing of virus Plaque forming assay
7. Dosing of virus Limiting dilutions
Autonomous laboratory work: isolation and characterisation of a wild bacteriophage.
Teaching Methods
1. Theoretical presentations
2. Bench working according predefined technical protocols
3. Oriented free laboratory work
4. Poster monograph presentations of a new invented virus.
The evaluation of the theoretical component, by continuous assessment of frequencies or final examination, will have a relative weight of 70%.
The practical component will be evaluated by the report of the work performed; it will have a relative weight of 15%.
The evaluation of the poster and its public presentation will have a relative weight of 15%.
If and when it is possible to do all teaching in the classroom, the relative weight of the monograph and the practical component will be reduced to 10% and 10% will be attributed to the attendance of students to all the components.
2. Bench working according predefined technical protocols
3. Oriented free laboratory work
4. Poster monograph presentations of a new invented virus.
The evaluation of the theoretical component, by continuous assessment of frequencies or final examination, will have a relative weight of 70%.
The practical component will be evaluated by the report of the work performed; it will have a relative weight of 15%.
The evaluation of the poster and its public presentation will have a relative weight of 15%.
If and when it is possible to do all teaching in the classroom, the relative weight of the monograph and the practical component will be reduced to 10% and 10% will be attributed to the attendance of students to all the components.
Certificações
