2024

Biotechnology

Name: Biotechnology
Code: BIO12356L
6 ECTS
Duration: 15 weeks/156 hours
Scientific Area: Biological Sciences

Teaching languages: Portuguese
Languages of tutoring support: Portuguese

Sustainable Development Goals

Learning Goals

1. Understanding Genetic Engineering as an integrated science from several other scientific areas, with
focus on a Biologic point of view
2. The teaching will be directed for the familiarization of techniques for the creation of added value from
new processes and products.
3. Based on previous knowledge on Molecular Biology, Genetics, Biochemistry, Immunology and Virology,
concepts for application of the new techniques will be discussed.
4. Application of Molecular Biotechnology on the Health); on the Environment; and on the Agriculture
(transgenic plants and animals for the food chain).
5. Laboratory work will be focused on the most common techniques used for the most recent
biotechnological applications.

Contents

1 Theoretical programme
a. Introduction
b. Fundaments of Molecular Cloning and DNA analysis
c. Microbial Systems in Genetic Engineering
d. Eukaryotic Systems in Genetic Engineering
e. Applications of Genetic Engineering for the development of new products and services
f. Official Regulation
2. Laboratorial programme
a. Training on pipeting. Solutions and dilutions.
b. Plasmidic DNA extraction (MiniPrep)
c. Restriction digestion of plasmidic DNA
d. Agarose gel electrophoresis
e. Preparation of competent bacteria
f. Transformation of competent bacteria
g. Screening of recombinants
h. Autonomous laboratorial work: Identification of unknown DNA sample

Teaching Methods

1 Theoretical presentations
. Bench working according with predefined technical protocols
. Oriented free laboratory work
. Monograph presentations as public posters

Teaching Staff (2023/2024 )